Hangzhou Powersonic Equipment Co., Ltd.
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20kHz Ultrasonic Liquid Processor for Cell Disruption Drug Extraction
Parameter
| Model | SONO20-1000 | SONO20-2000 | SONO15-3000 | SONO20-3000 |
| Frequency | 20±0.5 KHz | 20±0.5 KHz | 15±0.5 KHz | 20±0.5 KHz |
| Power | 1000 W | 2000 W | 3000 W | 3000 W |
| Voltage | 220/110V | 220/110V | 220/110V | 220/110V |
| Temperature | 300 ℃ | 300 ℃ | 300 ℃ | 300 ℃ |
| Pressure | 35 MPa | 35 MPa | 35 MPa | 35 MPa |
| Intensity of sound | 20 W/cm² | 40 W/cm² | 60 W/cm² | 60 W/cm² |
| Max Capacity | 10 L/Min | 15 L/Min | 20 L/Min | 20 L/Min |
| Tip Head Material | Titanium Alloy | Titanium Alloy | Titanium Alloy | Titanium Alloy |
Description
The purpose of breaking the cell is to damage the cell wall and cell membrane to varying degrees (increase permeability) or to break and release the target product. The main methods used are mechanical and non-mechanical.
Mechanical crushing has a large processing capacity, fast crushing speed, short time, and high efficiency. It is an important means for industrial-scale cell crushing. Cells are easily crushed by squeezing, shearing and impact. In many cases, cell content All are released. Because of the heat generated by mechanical stirring, cooling measures are required for crushing. The main methods of mechanical crushing are bead milling, high-pressure homogenization, impact crushing and ultrasonic.
In the separation and extraction of biological products, if the target substance is an extracellular product (such as glucoamylase produced by mold, etc.), the extraction process is relatively simple, and only solid-liquid separation is directly required to obtain a clear filtrate, and then the separation and purification operation; If the target substance is an intracellular product or various biomolecules in multicellular biological tissues (such as alkaline phosphatase and other intracellular enzymes, some exogenous gene expression industries or plant cell products, etc.), the cells or tissues need to be disrupted in advance , So that the target substance is fully released into the solution without losing biological activity.
Different organisms or tissues of different parts of the same organism have different procedures for cell disruption, and the methods used by cell disruptors are also different. For example, the cell membranes of animal organs are fragile and easy to be broken. Plants and microorganisms For cell walls composed of relatively strong cellulose and hemicellulose, special cell disruption methods must be adopted.
Application
Ultrasonic cell pulverizer can be used for scientific research in medicine. The research in medicine is mainly the extensive use of the function of crushing. This aspect has extremely high technical requirements.
Ultrasonic cell crusher can be used to crush animal, plant cells, bacteria, dental spores or tissues. Disperse rare earth and various inorganic mineral powders.
Ultrasonic cell crusher is an ideal device to accelerate the reaction speed of chemistry, biology and physics and accelerate the degassing of liquids.
Ultrasonic cell pulverizer can prepare latex with a size of nearly one hundredth of a micron; homogenize the "immiscible" mixture; polymerize some substances and precipitate others.
In short, the ultrasonic cell pulverizer can achieve multiple functions of extraction, pulverization, emulsification, homogenization, suspension, mutation, aerosol, accelerated desolvation, crystallization, and preparation of various biological samples under an electron microscope.
